Mourinho and Rose reportedly clashed after the left-back was left out of the squad for the 0-0 draw at Watford on Saturday, with young centre-half Japhet Tanganga filling in at left-back at Vicarage Road.
The Spurs manager explained afterwards that Rose had missed out with a back problem but sources confirmed the 29-year-old believed himself fit to play and was unhappy with Mourinho's decision.
Speaking ahead of Wednesday’s Premier League clash against Norwich, Mourinho insisted he had no issue with Rose and explained why he had missed Saturday's match and the midweek FA Cup win over Middlesbrough.
"A disagreement between me and Danny? No," Mourinho told a press conference. "My relationship with my players is good and I love to work with my group. And that's it.
"I don't know what you mean by tension in the air, I don't have any problem with him. He was not selected against Watford and Middlesbrough. The reason he was not selected against Middlesbrough is we have a young left-back we want to develop and it was a great opportunity to play [Ryan] Sessegnon. And also because [Danny's] performance against Liverpool was not phenomenal.
"On the Thursday before Watford, late evening, I got a call from my medical staff saying that Danny was calling them with a problem in his back and he wouldn't train the next day. So Friday, the day before the game, it was a bit of surprise that he was ready to train. It was not a big problem but even so I decided then to play with Tanganga and have Ryan on the bench. That's the situation.
"He has not had an issue with me," Mourinho added. "I receive a written report every day from my medical department, and it this case, because it was too late – 9-10pm – it was not a written report but a telephone call. My medical department know that it's very important for me to know for my next training session if my players are available.
"They called me to say that if I needed a left-back for my training session, it was better to call the academy and bring Dennis Cirkin because Danny Rose was not available to play."
Mourinho confirmed that Christian Eriksen would be involved against Norwich, as Inter Milan wait to discover if their latest bid for the Dane – believed to be £12.8million – has been accepted by Spurs.
The Portuguese will also welcome back club-record signing Tanguy Ndombele, who has not featured since the defeat at Southampton on New Year's Day due to a hip problem.
"He's ready, he's not ready for 90 minutes, he will be involved, he will be selected," Mourinho said of Ndombele. "I'm not telling you to start or come later, but no injury. Completely free. Just a question of condition and mentality to be ready, but he's back tomorrow.
"I am confident [he can have a run of games] but let's see. We know what he can give to the team. If I focus on the few minutes or matches that he played for us I saw qualities that are very important for a team to play like we do.
"But of course, it is not nice to play a game and after 15 minutes be out, it is not nice to play one game and then stops for two, three or four."