Police are investigating an alleged incident that occurred in the tunnel after Barnsley's 4-2 League One win over Fleetwood Town on Saturday.
A tweet from Barnsley striker Cauley Woodrow, which has since been deleted, suggested Fleetwood boss Joey Barton confronted opposite number Daniel Stendel.
"The club can confirm there was an alleged incident in the tunnel area that took place following the conclusion of today's match, which South Yorkshire Police are currently investigating," Barnsley said in a statement.
"The club is assisting the police with its enquiries and, as a result, we are not in a position to make any further comment at this time."
Sky Sports News showed footage after the match that appeared to show Barton attempting to leave the ground in a car before being stopped by police.
A South Yorkshire Police statement read: "Officers received reports of an assault at Barnsley Football Club today, Saturday 13 April, this incident will be thoroughly investigated."
Neither Barnsley nor Fleetwood fulfilled their post-match media duties after the game, where victory moved the Tykes back into the second automatic promotion place.