Two of Europe's heavyweight clubs were drawn together in Group G, with Barca and Juve both getting off to winning starts against Ferencvaros and Dynamo Kiev respectively last week.
The defining on-pitch rivalry of the modern era - Messi vs Ronaldo - was set to reignite for the first time since May 2018 before Ronaldo left Madrid for Turin.
However, Ronaldo contracted coronavirus earlier this month, and it looks as though he will not be cleared to play.
Messi and Barca will be looking to bounce back from Saturday's disappointing 3-1 Clasico defeat at home to Ronaldo's former club Real Madrid.
Date, time and venue
Juventus vs Barcelona is scheduled for an 8pm BST kick-off tonight (Wednesday, October 28, 2020).
The match will be behind closed doors at Juventus Stadium in Turin, with no fans due to be present due to government guidelines regarding coronavirus.
How to watch
TV channel: The match will be televised on BT Sport 3, with coverage starting from 8pm.
Federico Chiesa will be available given his recent suspension was a domestic one, while Matthijs de Ligt is on the comeback trail following a shoulder injury.
Marc-Andre ter Stegen remains sidelined for Barca, meaning Neto should again play in goal.
Samuel Umtiti will also be out with a knee injury. With Antoine Griezmann and Ouzmane Dembele not starting the Clasico, they could come in for Ansu Fati and Pedri.
Barca will almost certainly be without Philippe Coutinho because of a hamstring injury the midfielder sustained in the Clasico.
Prediction: Juventus 1-1 Barcelona
Given it is still early in the season and neither side is firing on full cylinders, a tense draw could be on the cards - unless Messi or Ronaldo step up, that is.
Head to head (h2h) history and results
The two sides have faced each other 11 times in Uefa competitions: Barca edge it with four wins to Juve's three, along with three draws.
Barca got the better of Juve in the 2017/18 group stages, winning 3-0 at home and drawing 0-0 in Turin.
Before that, Juve saw off Barca 3-0 on aggregate in the 2016/17 quarter-finals.
The biggest recent meeting between the sides saw Barca beat Juve 3-1 in the 2014/15 Champions League Final.
Messi vs Ronaldo head to head
In the 35 matches directly involving the pair, Messi has won 16 games to Ronaldo's 10.
Messi has also scored more goals than Ronaldo, with 22 to 19 - and when it comes to the Champions League, Ronaldo is yet to score against a Barcelona side featuring Messi.
Betting odds and tips
Juventus to win: 2/1
Barcelona to win: 8/5
Over 2.5 goals: 8/11
Odds courtesy of Betfair Exchange. Click here to find out more.
This weekend get a £10 free bet with Betfair, when you bet £10 on a Same Game Multi on the Premier League.
Terms: Min £10 Same Game Multi bet on any EPL match this Fri - Sun. Free bet valid for 72 hours, awarded at bet settlement. Excludes cashed out bets. T&Cs apply.
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