Jeanine Anez Chavez tweeted the news on Thursday evening.
It comes just days after Brazil's president said his Covid-19 test had come back positive.
Ms Anez says she was "well" and would keep working from isolation.
The country's health minister, Maria Eidy Roca, recently said she had tested positive positive for the virus.
He dado positivo a Covid19, estoy bien, trabajaré desde mi aislamiento. Juntos, vamos a salir adelante. pic.twitter.com/oA4YVYlZFa— Jeanine Añez Chavez (@JeanineAnez)July 9, 2020
Bolivia, which has a population of more than 11.5 million people, has registered more than 42,000 confirmed cases of the disease and 1,500 deaths.
It is one of the worst affected countries per capita in the world.