Alba missed last weekend’s defeat at Getafe with a hamstring problem but returned to training on Thursday and starts, with summer signing Sergino Dest also involved.
Sergio Busquets was rested for the midweek Champions League win over Ferencvaros and returns at the expense of Miralem Pjanic.
Antoine Griezmann has struggled to find form under Koeman, and the coach has gone with Lionel Messi, Ansu Fati and Pedri instead.
No1 goalkeeper Marc-Andre ter Stegen remains out after undergoing surgery on a tendon problem in August, while Samuel Umtiti is also missing because of a persistent knee problem.
The Catalan side are ninth in LaLiga, three points adrift of their third-placed rivals, but with a game in hand.
Barcelona XI: Neto, Dest, Lenglet, Pique, Jordi Alba, De Jong, Busquets, Coutinho, Messi, Pedri, Ansu Fati
Real Madrid XI: Courtois, Nacho, Ramos, Varane, Mendy, Casemiro, Kroos, Valverde, Vinicius, Asensio, Benzema
Subs: Lunin, Luis Lopez, Militao, Modric, Marcelo, Lucas, Jovic, Isco, Rodrygo
This weekend get a £10 free bet with Betfair, when you bet £10 on a Same Game Multi on the Premier League.
Terms: Min £10 Same Game Multi bet on any EPL match this Fri - Sun. Free bet valid for 72 hours, awarded at bet settlement. Excludes cashed out bets. T&Cs apply.