Coach Quique Setien is without midfielders Sergio Busquets and Arturo Vidal, who are both suspended for the Camp Nou clash.
Antoine Griezmann, who scored for Barca in the sides’ 1-1 draw in the first leg back in February, starts after recovering from a muscle injury which saw him miss the end of LaLiga.
Lionel Messi and Luis Suarez start with Ansu Fati on the bench, but Ousmane Dembele is not quite ready after a long-term injury and is not in the squad.
Dembele has been out since November and is closing in on a return to action, with Setien saying on Friday that he will feature if Barcelona make it through to the final phase in Portugal next week.
Striker Martin Braithwaite, signed in February in an emergency deal as Dembele was ruled out for a further six months, is ineligible for the Champions League.
Meanwhile, midfielder Arthur is back in Barcelona after telling the Catalan club he had planned to stay in Brazil, but the 23-year-old will play no part in this match and is unlikely to represent the Blaugrana again as he seeks to rescind his contract before joining Juventus in the summer.
Samuel Umtiti is still sidelined, but fellow centre-back Ronald Araujo is back on the bench after suffering an injury playing for Barca B in their recent promotion play-off defeat to Sabadell.
American winger Konrad de la Fuente is in the senior squad for the first time and the 19-year-old is on the bench at Camp Nou, along with a number of other young players.
Barcelona XI: ter Stegen; Semedo, Pique, Lenglet, Jordi Alba; Rakitic, Roberto, De Jong; Messi, Suarez, Griezmann
Subs: Neto, Junior Firpo, Inaki, Ansu Fati, Puig, Araujo, Monchu, Mingueza, Konrad, Reis, Orellana