Barca were held in their opening match – a goalless draw with Borussia Dortmund in Germany – but have since beaten both Inter Milan and Slavia 2-1.
However, they will tonight be without Luis Suarez, who suffered a calf injury in the club’s recent 3-1 defeat to Levante.
The Uruguay international is expected to be out for the next three weeks.
Slavia are meanwhile still hunting for their first win in this season’s tournament. They began in encouraging fashion, holding Inter to a 1-1 draw in Milan, but have since lost to both Dortmund and Barcelona. Here is everything you need to know.
When is the match?
The match is today: Tuesday 5 November.
What time does it kick-off?
The match gets underway at 5.55pm.
Where can I watch the match?
The match will be shown live On BT Sport 1.
BT Sport subscribers will also be able to stream the match online via the video player and BT Sport app.
What are the expected line-ups?
Barcelona possible XI: Ter Stegen; Semedo, Pique, Lenglet, Alba; Busquets, De Jong, Vidal; Messi, Griezmann, Dembele
Slavia Prague possible XI: Kolar; Coufal, Kudela, Frydrych, Boril; Sevcik, Soucek, Zeleny; Stanciu; Olayinka, Masopust
What is our prediction?
Barcelona to bounce back from their La Liga loss with a comfortable 2-0 victory.