The Kazakh side pushed United close at Old Trafford, with only a late Mason Greenwood winner separating the two sides.
The visitors bring a particularly young squad, with Ole Gunnar Solskjaer‘s team already assured of a place in the next round of the competition.
As many as ten of the travelling party could make first-team club debuts, while Lee Grant may start in goal.
Here is everything you need to know about the Europa League clash.
Manchester United 19/20
A young Manchester United side may find it tough going against Astana, who are a better side than their Europa League record suggests. 1-1 is our prediction.
With the busy festive schedule fast approaching and progression out of the group stages already secured, Ole Gunnar Solskjaer is set to rotate his squad. Both David De Gea and Sergio Romero have not travelled, which means Lee Grant is in line for a first start in goal.
Ten of the travelling squad are in line for a first team debut in Kazakhstan, with young centre-half Teden Mengi set for a starting berth. Luke Shaw may return at left-back after recovering from injury.
Manchester United: Grant, Laird, Bernard, Tuanzebe, Shaw, Garner, Levitt, Chong, Lingard, Gomes, Greenwood.
When is it?
Kick-off at the Astana Arena is 3.50pm GMT on November 28.
How can I watch it?
The match will be shown live on BT Sport 2, with coverage beginning at 3.30pm.
BT Sport customers can watch the match via the BT Sport app or on the online player.